Gabriel Barbosa helped Santos continue their strong form in the Copa Libertadores, while Atletico Nacional won comfortably on Tuesday.
Gabriel, on loan from Inter, opened the scoring in Santos' 2-0 win over Estudiantes in Sao Paulo.
Returning from suspension, Gabriel got on the end of a long pass from Jonathan Copete before finishing clinically just before half-time.
Santos doubled their lead early in the second half, Lucas Verissimo heading in a Jean Mota set-piece.
The win moved Santos onto nine points in Group 6, five clear of second-placed Estudiantes.
Atletico Nacional cruised to a comfortable 4-1 win over Bolivar in Group 2 in Medellin.
Gonzalo Castellani scored a scrappy opener before Atletico struck twice after the half-hour mark, Vladimir Hernandez and Dayro Moreno putting away rebounds.
While Marcos Riquelme tapped in a goal for Bolivar shortly after half-time, Moreno sealed Atletico's win.
The Colombian side made it three wins in four in Group 2, moving four points clear of Bolivar.
In Group 7, a brace from Carlos Sierra helped Deportivo Lara edge Millonarios 2-1.